Measurement
M_PTI1

Part:BBa_K5088500:Design

Designed by: Dascha Khalfine   Group: iGEM24_Marburg   (2024-07-31)


Measurement construct - PTI1 promoter+5'UTR from T. kok-saghyz


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1266
    Illegal XbaI site found at 188
    Illegal XbaI site found at 3537
    Illegal PstI site found at 3964
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1266
    Illegal PstI site found at 3964
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1266
    Illegal BglII site found at 413
    Illegal BglII site found at 3540
    Illegal XhoI site found at 440
    Illegal XhoI site found at 2784
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1266
    Illegal XbaI site found at 188
    Illegal XbaI site found at 3537
    Illegal PstI site found at 3964
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1266
    Illegal XbaI site found at 188
    Illegal XbaI site found at 3537
    Illegal PstI site found at 3964
    Illegal NgoMIV site found at 3012
    Illegal AgeI site found at 1765
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3226


Design Notes

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Source

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References